Vero cell line is a continuous cell line derived from the kidney of an African green monkey, widely used in microbiology, molecular biology, and cell biology research.
Understanding Vero Cell Lines
Vero cells are a staple in biological research due to their ability to support the growth of viruses and their adaptability to various experimental conditions. Here's a more detailed look:
Origin and Characteristics
- Source: Originally derived from the kidney of an African green monkey.
- Type: Continuous cell line, meaning they can divide indefinitely under proper conditions. This is crucial for consistent and reproducible research.
- Application: Common in virology, immunology, and vaccine production.
Common Uses in Research
- Virus Propagation: Vero cells are highly susceptible to many viruses, making them ideal for virus isolation, amplification, and titration.
- Cytotoxicity Assays: Used to assess the toxic effects of various substances on cells.
- Vaccine Production: Vero cells are used to grow viruses for vaccine production, especially for diseases like polio and influenza.
Culturing Vero Cells
The referenced material includes protocols for growth and maintenance of Vero cell lines in a research laboratory setting, showing their utility in various researches.
| Aspect | Description |
|---|---|
| Growth Medium | Typically requires specific growth media supplemented with serum and antibiotics to maintain cell viability and prevent contamination. |
| Incubation | Usually maintained at 37°C in a humidified atmosphere with 5% CO2. |
| Passaging | Cells are passaged (split and diluted) regularly to prevent overgrowth and maintain healthy cultures. |
| Storage | Can be cryopreserved in liquid nitrogen for long-term storage. |
