Xenium 10x refers to Xenium, a cutting-edge, high-resolution, imaging-based in situ spatial profiling technology developed by 10x Genomics. This innovative platform is designed for the simultaneous expression analysis of hundreds of RNA targets directly within an intact tissue section, allowing researchers to understand the precise location and context of gene activity.
The Xenium platform from 10x Genomics represents a significant advancement in the field of spatial biology. Traditional molecular analysis often requires breaking down tissue into individual cells, which leads to the loss of crucial spatial information about where genes are expressed within the tissue's complex architecture. Xenium overcomes this limitation by enabling researchers to visualize and quantify RNA molecules while preserving the original tissue morphology.
Key Features of Xenium 10x
- High-Resolution Imaging: Xenium provides highly detailed images, allowing for the precise localization of RNA transcripts within cells and across tissue structures. This high resolution helps in understanding subcellular processes and interactions.
- In Situ Spatial Profiling: The technology analyzes gene expression directly "in place" within biological tissues. This means that the spatial relationships between different cells and their expressed RNA targets are maintained, which is vital for studying complex biological systems like tumors, the brain, or developing embryos.
- Simultaneous Multi-Target Analysis: Xenium is capable of simultaneously detecting and quantifying a large number (currently in the range of hundreds) of specific RNA targets within a single tissue section. This multiplexing capability provides a comprehensive molecular snapshot of the tissue's functional state.
- Developed by 10x Genomics: As a product of 10x Genomics, a leader in single-cell and spatial genomics technologies, Xenium leverages advanced tools and expertise to provide robust and reliable data.
Applications and Significance
The ability of Xenium 10x to analyze gene expression with spatial context is invaluable for various research areas, including:
- Cancer Biology: Understanding the heterogeneity of tumor cells and their interactions with the surrounding microenvironment.
- Neuroscience: Mapping gene expression patterns in brain regions to understand neurological diseases and brain function.
- Developmental Biology: Tracing gene activity during embryonic development and tissue formation.
- Immunology: Characterizing immune cell states and their spatial distribution in tissues during infection or autoimmune diseases.
By providing a detailed molecular map of biological tissues, Xenium 10x empowers scientists to gain deeper insights into disease mechanisms, identify novel biomarkers, and develop more targeted therapies.
